Journal: Chemical Science

Article Title: Bioinspired nonheme iron complex that triggers mitochondrial apoptotic signalling pathway specifically for colorectal cancer cells †

doi: 10.1039/d1sc05094j

Figure Lengend Snippet: DCFDA assay for the detection of intracellular ROS produced in response to oxidative stress caused by DW, L, [M(HN 3 O 2 )] 2+ (M(L); M = Mn, Fe, Co, and Cu), vehicle, and tert -butylhydroperoxide (TBHP) in (a) HCT116 and (b) CCD-986Sk cells. Cells were treated with 50 μM of metal complexes for 24 h and with 50 μM of TBHP alone for 4 h as the positive control. Data are shown as mean fold change (FC) to control ± standard error of the mean (SEM). The statistical analysis was performed using one way ANOVA-Dunnett's test and t test. Statistical significance is shown as: * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = nonsignificant as compared to control ( n = 3).

Article Snippet: ROS were detected using 2′,7′-dichlorofluorescin diacetate (DCFDA) cellular ROS detection assay kit (Abcam # ab113851) following the manufacturers' protocol.

Techniques: Produced, Positive Control

Journal: PLOS ONE

Article Title: Clinical outcomes and anti-inflammatory mechanisms predict maximum heart rate improvement after physical activity training in individuals with psychiatric disorders and comorbid obesity

doi: 10.1371/journal.pone.0313759

Figure Lengend Snippet: MHR: maximum heart rate, HRSD: Hamilton rating scale for depression, PANSS-P: positive and negative syndrome scale (positive subscale), PANSS-G: positive and negative syndrome scale (general subscale), IL-10: interleukin 10, TNF-α: tumor necrosis factor alpha, ROS: reactive oxygen species; ΔΨm: mitochondrial membrane potential, VCAM: vascular cellular adhesion molecule, LEPMN: leukocyte-endothelium adhesion polymorphonuclear, RVPMN: rolling velocity polymorphonuclear cells, PSEL: P-selectin; MPO: myeloperoxidase.

Article Snippet: In total, 100 000 cells were plated in each well of 96-well plates and incubated for 30 min at 37°C with the corresponding fluorochromes, as follows: dichlorofluorescein diacetate to measure reactive oxygen species (ROS) production (485 nm excitation, 535 nm emission), MitoSOX to measure mitochondrial ROS (mROS) (510 nm excitation, 580 nm emission), tetramethylrodamin methyl ester to assess mitochondrial membrane potential (ΔΨm) (552 nm excitation, 574 nm emission), superoxide dismutase (SOD) (total SOD/T-SOD Activity Assay Kit–Colorimetric—from NOVUS), and 5-chloromethylfluorescein diacetate to measure intracellular glutathione (GSH) (492 nm excitation, 517 nm emission).

Techniques: Membrane